About 800,000 people in Japan are suffering from thinning hair. To provide hope, a new hair loss treatment is emerging. The black hair on the nude mice shown here are not implanted or grafted. They are being regenerated using a regenerative therapy technique. Regenerative therapy is used for hair loss treatment. iPS cells are used as well. These are iPS cells in the middle of differentiation induction. Hair treatment using cutting edge science is emerging. Thanks to the new regenerative medicine technology, we can regenerate hairs from cells. Hair generation starts in a very small organ present in the dermis.
To generate hair, appropriate cells are needed. Cell to cell interaction is important. We need to focus on recreating the natural hair regenerative process as accurately as possible. Today we are showing the science behind hair regeneration what is happening at a cellular level in the scalp. Science ZERO Japan It’s exciting! Hair treatment using cell therapy. I’d love to try it myself! I think a lot of people are interested in learning about this new therapy. Do you know how your hairs grow and shed All I know is that hair grows from hair follicles, but I don’t know much abut the mechanism.
To understand the mechanism of hair growth, you need to know what is going on inside hair follicles. The structure you see here is called a hair follicle. The hair follicle functions like a ‘hair regeneration factory’. It can be divided into 2 compartments. The outer part of the hair follicle envelopes a hair fiber. The other part inner compartment of the hair follicle is a collection of important cells called dermal papilla cells. Dermal papilla cells instruct hair matrix cells to replicate. They are instructed to push cells to grow upward, inducing them to differentiate into a hair shaft and elongating hair fiber.
Shiseido Talks RepliCel Technology Part 1
Hair is a group of cells which are pushed upward after cell division. That’s right. This is a tutorial clip that shows you how hair grows. Wow, amazing. It looks as if the hair is physically being pushed up. The question we would like to ask here is why we lose hair. Let’s ask scientists about it. Professor Akio Sato from Kitasato University School of Medicine It is very important to understand hair cycle when we talk about thinning hair. A hair cycle is a growth cycle of hair. Hair cycle has three different cycles.
The first one is called Anagen phase, which lasts for two to six years. The next phase is called Catagen phase, at which cell division slows down. The last phase is called Telogen phase, which is a resting stage where no cell division occurs. The hair is shed at the end. Are the cycles repeated Yes, since Anagen phase is the longest of all 3 cycles, hair always appears thick. I understood that hair thinning occurs when the hair cycle is disrupted. But what causes this disruption Typically, it is thought that thinning is caused by the effects of a male hormone, called androgen. Through an enzymatic reaction, this male hormone is converted into a more active form.
This form is believed to have detrimental effects on the hair cycle such an inhibition of cell division. The cells appear unhealthy here. In this case, a Catagen phase comes early, shortening the hair cycle. And the shortened cycle is repeated. Before hair has a change to be elongated, this short cycle ends, and a new short cycle begins, resulting in thinning hair. For healthy hair regeneration, it needs enough time to grow. When you have a close look at the scalp of thinned hair, you can see a lot of short hairs.
You’re right. There are lots of short hairs. It’s not completely bald! Hair follicles in the scalp of the people with thinning hair are not dead. Dermal papilla cells stop sending stimulatory signals, shortening the hair cycle. This is why hair appears thinner. What’s the scoop on current hair loss treatment There is a medical product in the market that antagonizes the male hormone. The drawback of this product is that people have to continuously take it to have lasting effects. However, there is a new therapy under development that has potential to stimulate dermal papilla cells to promote hair regeneration.
This is Shiseido’s research center in Yokohama, Japan. The hair research team at Shiseido is focusing on development of a cellbased regenerative medicine technology for hair loss. Project leader, Dr. Jiro Kishimoto, is a hair research expert, with more than 20 years of experience. We start the process by isolating hair follicles from a scalp sample. These are human scalp samples provided for research purposes after surgical procedures. They are investigating the effects of a specific type of cells within the hair follicle, called dermal sheath cup cells. Dermal sheath cup cells are located beneath or surrounding the dermal papilla.
Dermal sheath cup cells hold the dermal papilla cells inside. Dermal sheath cup cells are considered to grow to be the dermal papilla cells. Dermal sheath cup cells envelope dermal papilla cells and are believed to stimulate dermal papilla cells to release factors which are important in hair regeneration and elongation of hair fibers. In other words, dermal sheath cup cells are very important in hair growth as they activate dermal papilla cells, which results in a longer Anagen phase. This is an image of a mouse which received injection of dermal sheath cup cells only in one ear.
This image was taken 6 months after the injection. You can clearly see that the right ear which has the cell injection has more hair growth than the left ear. Jiro and his team at Shiseido are trying to apply this technology to humans. This is the procedure. A biopsy is taken from the back of the scalp, which is typically resistant to the hormone that causes baldness. Dermal sheath cup cells are isolated and cultured until enough cells are obtained. These cells are then injected into the patient’s scalp. It is expected that the injected dermal sheath cup cells would stimulate residing dermal papilla cells, resulting in a longer Anagen phase and thicker hair.
You may call this technology the ‘rejuvenation of hair follicles’ in the sense that we are ‘reactivating’ hair follicles back to a healthier state. Let’s take a look at the actual cell preparation procedures. First, they use a scalp biopsy to isolate hair follicles. The small detached section on the right is the important part of this technology. When you invert this structure inside out, the top oval structure is a group of dermal papilla cells. The bottom mushroomshaped structure is a group of dermal sheath cup cells. It is only 0.3mm big.
After isolation of dermal sheath cup cells, the cells are cultured in a proprietary culture medium, which is formulated such that cells grown in it maintain hair regenerative properties. Cultured cells are injected back into the patient’s scalp. These injected cells are believed to migrate close to residing dermal papilla cells. Dermal papilla cells, which come in close contact with the injected dermal sheath cup cells are stimulated, leading to regeneration of hair. As a result, an Anagen phase gets longer, which promotes hair growth. I hope that this new technology will provide a hope to those people who have been suffering from thinning hair.
The Shiseido team has a cell manufacturing facility in Kobe and is planning to initiate a clinical research study soon. They are hoping to market this therapy for clinical use by 2018. It’s quite an interesting idea to inject one’s own cells into the scalp. I’d like to try the treatment myself. What is the treatment process like Patients go to the clinics, and biopsies are taken from their scalp. The biopsy is then sent to a cell manufacturing facility where the cells are isolated and cultured. After that, the cells are sent back to the clinic, at which point a doctor injects the cells back to the same patients.
Biopsies are taken from the back of the scalp. Why When you take a look at people with thinning hair, most people still have a fair amount of hair at the back of the scalp, but not on the top or on the sides. The cells at the back of the scalp are resistant to the effects of a male hormone. The key to this technology is to isolate and culture these hormoneresistant cells and put them back to the affected area to stimulate hair growth. Currently available drugs are mainly targeted for men, and a limited treatment is available for women. The good news is that this new technology by Shiseido will be useful for women as well.
Are the hair stimulating effects expected to last because cells are injected We assume that the injected cells migrate close to residing dermal papilla cells and continue to have stimulating effects. I would love to treat patients once this hair loss treatment is established. I’m sure this helps to reduce the number of people with hair lossthinning hair problems in the future. This technology however, can only apply to those who have intact hair follicles. There are some people who have lost their hair follicles due to health conditions. Health conditions such as such as hereditary conditions or as a result of injuries, burns or the use of anticaner drugs. This technology can’t be applied to those people.